Single crystal X-ray diffraction is a non-destructive analysis technique that can reveal important details about the internal lattice of crystalline materials, such as unit cell size, bond length, bond angle, and site ordering. X-ray or electron diffraction techniques can be used to determine the three-dimensional structure of a chemical or biological component as it crystallizes into a single crystal. This technology gives accurate and precise molecule size measurements in a way that other approaches cannot. It's frequently used to detect natural compounds or the results of synthetic chemical research, but it can also be used to investigate exact molecule geometry, intermolecular interactions, and absolute configurations.
Alfa Chemistry has a variety of single crystal X-ray diffraction instruments in our analytical laboratory that can run samples under restricted sample preparation circumstances. Please contact Alfa Chemistry for more information about our single crystal X-ray diffraction laboratory.
The technique of X-ray diffraction is currently widely used to analyze crystal structure and atomic spacing. The constructive interference of monochromatic X-rays and crystal samples is the basis of X-ray diffraction. A cathode ray tube produces the X-rays, which are subsequently filtered to produce monochromatic radiation, collimated and concentrated, and directed onto the sample. The interaction between the incident light and the sample will create constructive interference when the condition satisfies Bragg's law (n λ = 2 d sin θ). The wavelength of electromagnetic radiation is related to the diffraction angle and lattice spacing in a crystal sample by this law. The diffracted X-rays are then identified, processed, and tallied. All conceivable lattice diffraction orientations can be obtained by modifying the geometry of the incident light, the direction of the center crystal, and the detector.
Crystal structure determination, molecular structure and geometric shape, absolute structure and configuration, single crystal diffraction, powder diffraction, environmental and non-environmental conditions, charge density, intermolecular interactions, and structure correlation are some of the applications of single crystal diffraction.
A 2.5 X-ray source, D85 four-circle kappa goniometer, the most sophisticated multilayer optics, and an Apex II CCD area detector are all included in the instrument. The device is equipped with a liquid nitrogen-based cryogenic system that can capture data at temperatures ranging from 80 to 500 degrees Celsius.
A dual-port copper rotating anode generator, each with confocal optics, a "desktop beamline" with a Mar 345 image plate detector, and a liquid nitrogen-based crystal Cooling mechanism make up the polymer single crystal X-ray diffractometer. Inside macromolecules, the system can assess their structure with atomic resolution and select samples that require a synchrotron source.
The instrument features a large CCD area detector set atop a four-circle goniometer and a choice of copper or molybdenum X-ray sources. Shorter wavelength molybdenum sources can offer improved resolution and lessen absorption effects. The longer-wavelength copper source is more intense, which aids in determining the light atom structure's absolute structure. For cryogenic data collecting, the equipment is fitted with a cryogenic system based on liquid nitrogen.
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