Raman spectroscopy is a non-destructive chemical analysis technique that uses light scattering to reveal details about chemical structure, crystallinity, and molecular interaction. Light from a high-intensity laser light source is scattered by the molecules. The wavelength of most dispersed light is identical to that of the laser source, and it provides little helpful information (Rayleigh scattering). However, Raman scattering occurs when a little amount of light is dispersed at different wavelengths depending on the chemical structure of the analyte.
Multiple peaks in the Raman spectrum indicate the strength and wavelength position of Raman scattered light. Each peak represents a different chemical bond vibration, including single bonds like CC and C=C, as well as bond groups like benzene ring breathing mode, polymer chain vibration, and lattice mode. Raman spectroscopy identifies the chemical structure of materials and offers information on chemical structure and properties, phase and polymorphism, internal stress/strain, pollution, and contaminants, among other things.
The Raman Microspectroscopy Laboratory at Alfa Chemistry is our primary research facility for the quick, non-destructive characterization and chemical imaging of a wide range of materials, including minerals, biological samples, fluids, and dissolved gases. Our equipment is designed to acquire entire Raman spectra and is tailored for quick hyperspectral imaging of complicated samples and materials. This technique, when used in conjunction with modern multivariate statistical techniques, aids in the detection and identification of all Raman active chemicals present in the study area.
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From simple point spectroscopy to more sophisticated studies, the equipment can handle them all. Multiple lasers produce excitation lines ranging from visible light to near-infrared (488, 514, 633, 785, and 830 nm), and can be configured to meet a variety of experimental needs.
The system is linked to an Atomic Force Microscope (AFM) and can map micro-and nanoscale attributes using AFM and Tip Enhanced Raman Spectroscopy (TERS).
A 1W Nd: YAG air-cooled laser provides an excitation wavelength of 1064 nm for the apparatus. For studying substances that fluoresce when excited by other high-energy visual excitation lines, NIR excitation is particularly beneficial.
Its high-throughput module can be set up for studies with sampling geometries of 90 or 180 degrees. Sample analysis on a microscopic scale is possible thanks to fiber coupling to the microscope, which enables a spatial resolution of 8 m. High-throughput optics and germanium diode detectors ensure great sensitivity by detecting ultra-low signals with minimum noise. The software can control analysis settings, such as laser power and sample position, using real-time spectrum display.
The bioanalyzer is a portable, user-friendly Raman imaging system designed specifically for biological sample analysis. The system acquires high-resolution Raman images in order to provide quick and detailed information on the distribution and concentration of biochemical substances in biological samples (such as tissue biopsies, tissue sections, and biological fluids). The method is non-invasive, requires no staining or labeling, has great specificity, and can simultaneously assess numerous molecular components in a biological sample.
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